Journal: Journal of Neuroinflammation

Article Title: Targeting glial fibrillary acidic protein in glaucoma: a monoclonal antibody approach to modulate glial reactivity and neuroinflammation for neuroprotection

doi: 10.1186/s12974-025-03482-8

Figure Lengend Snippet: Microarray analysis reveals the effects of GFAP mAb treatment on key inflammatory mediators in glaucomatous retina. ( A - C ) The expression of TLR4 and S100A8 was significantly downregulated by 25–50 µg GFAP mAb treatment compared to the vehicle. The marker for microglial activation, CD68, was significantly decreased in the 25 µg GFAP mAb treatment group. ( D - F ) Proteins of the inflammasome pathway, NLRP3, GSDMD and Caspase-1 were significantly downregulated in the 25 µg GFAP mAb treatment group. With the 50 µg dose, these protein expressions showed a similar downward trend, but without statistical significance. ( G ) Expression profiling of inflammation-associated mediators showed that 25 µg GFAP mAb significantly decreased pro-inflammatory factors (TNF-α, IL-1β, IL-8, MMP9, and IFN-γ), and increased the anti-inflammatory cytokine IL-10 compared to the vehicle group. In contrast, the 50 µg dose significantly reduced IFN-γ only. ( H ) Representative images of spots showing IL-1β levels in the subarrays for each group. Image analysis was conducted with Imagene software, using the median intensity from each spot to calculate the mean of the triplicate spots for each marker. For CD68 and TLR4, due to significant heterogeneity in SDs, statistical analysis was performed using Welch’s ANOVA followed by the Tamhane T2 post hoc test. All other data were analyzed using one-way ANOVA with Tukey’s post hoc test. Data are presented as the mean ± SD; n = 4 per group; * p < 0.05, ** p < 0.01, *** p < 0.001; ns = not significant. TLR4 – Toll-Like Receptor 4. S100A8 – S100 Calcium Binding Protein A8. NLRP3 – NLR Family Pyrin Domain Containing 3. GSDMD – Gasdermin D

Article Snippet: Briefly, the microarray was prepared using a noncontact microarray printer (SciFLEXARRAYER S3; Scienion, Berlin, Germany).

Techniques: Microarray, Expressing, Marker, Activation Assay, Software, Binding Assay

Journal: Advanced Functional Materials

Article Title: Nanoporous, Gas Permeable PEGDA Ink for 3D Printing Organ‐on‐a‐Chip Devices

doi: 10.1002/adfm.202315035

Figure Lengend Snippet: Figure 6. Coculture of cancer and stromal cells in the OoC device. a) 3D confocal microscopy images of tdTomato+ MDA-MB-231 spheroid co-cultured with GFP+ IMR-90 cells at different time points in the OoC platform. b) Depth color map for the height range of 400 μm showing bi-directional migration of cells. c) Confocal microscopy images of GFP+ MDA-MB-231 breast cancer cells spheroid co-cultured with mCherry+ HUVEC cells, demonstrating vessel formation and integration of the cancer spheroid into the vasculature (white and yellow arrows, respectively). d) Schematic of microarray-based proteomic analysis and CXCL12 secretion under different culture conditions, showing increased concentration for OoC co-culture that induces cell migration. Three biological repeats and 20 technical repeats were done for each condition and time course.

Article Snippet: Protein Microarray for CXCL12 Quantification: The CXCL12 antibody microarray was fabricated onto 2D-Aldehyde glass slides (PolyAn, Germany) using a sciFLEXARRAYER SX microarray spotter (Scienion) equipped with a single piezo dispense capillary nozzle with coating 1 (Scienion).

Techniques: Confocal Microscopy, Cell Culture, Migration, Microarray, Concentration Assay, Co-Culture Assay